Red Clover Extract Powder

Parameter	Specification
CAS No.	485-72-3 (Formononetin)
Molecular Formula	C16H12O4
Molecular Weight	268.26
Active Ingredient	Total Isoflavones
Specifications	4:1, 10:1, 8%, 20%, 40% (HPLC)
Appearance	White to light beige powder
Particle Size	80–200 mesh
Test Method	HPLC, HPTLC
Moisture Content	≤5.0%
Solubility	Ethanol, propylene glycol, hot water
Test Item	Standard Requirement	Test Result	Judgment
Appearance	White to light beige powder, uniform color, no visible foreign impurities	White to light beige powder, clean and uniform	Complies
Odor & Taste	Mild characteristic herbal odor, slightly bitter taste	Conforms to typical sensory properties	Complies
Identification (TLC)	Thin-layer chromatogram matches Trifolium pratense reference herb	Positive identification	Complies
Total Isoflavones (HPLC)	≥8.0%	8.41%	Complies
Loss on Drying	≤5.0%	2.76%	Complies
Total Ash	≤8.0%	4.23%	Complies
Acid Insoluble Ash	≤2.0%	0.79%	Complies
Particle Size	100 mesh pass rate ≥98%	99.6%	Complies
Solubility	Partially soluble in water, soluble in ethanol solution	Conforms to solubility property	Complies
Lead (Pb)	≤2.0 ppm	0.40 ppm	Complies
Arsenic (As)	≤2.0 ppm	0.22 ppm	Complies
Cadmium (Cd)	≤0.3 ppm	Not Detected	Complies
Mercury (Hg)	≤0.1 ppm	Not Detected	Complies
Pesticide Residues	Comply with food safety maximum residue limits	Complies	Complies
Total Aerobic Microbial Count	≤1000 CFU/g	53 CFU/g	Complies
Yeast & Mold Count	≤100 CFU/g	Not Detected	Complies
Escherichia coli	Negative per gram	Negative	Complies
Salmonella spp.	Negative per 25g	Negative	Complies
Staphylococcus aureus	Negative per gram	Negative	Complies

Raw Material Excellence: Strict origin control and selection of flowers and leaves picked at their optimum bloom from our Xinjiang, Gansu and Northeast China growing bases to optimize the isoflavone content. Premium Dietary Grade (mold-free, pesticide-screened) Economical Crude Grade (cost-effective trimmings) Organic Grade (USDA/EU certified, segregated production lines) The materials are then dried at a low temperature (40–45°C) to retain the active chemicals.

R&D and testing facilities at the forefront of technology: In our laboratory, we use UPLC, HPLC, ICP-MS, GC-MS, HPTLC for the purpose of testing authenticity and quantification of isoflavones, heavy metals, pesticide residues and microbiological limits. Each batch is supplied with COA, MSDS and 3rd party testing assistance (SGS/Eurofins).

Scalable Production: We have 3 dedicated lines and 2,000+ tons yearly capacity to meet the requests from pilot batches to bulk supply with consistent quality and on-time delivery.

Origin & Grading:

Premium Dietary Grade: Fresh, delicate peak-bloom flowers/leaves; no mold, no insect damage, no dirt; extensively checked for pesticides and heavy metals; utilized for 8%/20% standardized extracts.

End of season materials and trimmings Economical Crude Grade Low cost Suitable for 4:1 aqueous extracts and herbal blends

Organic Grade: Made from chemical-free bases, USDA/EU organic certified, processed on separated lines to avoid cross-contamination.

Main Components: Isoflavones total, polysaccharides, saponins and phenolic acids.

Solubility:

Native Extract: Soluble in ethanol, propylene glycol and hot water; may create cloudiness due to sluggish dissolving in cold water. Almost insoluble in fats/oils – Must be pre-dissolved in polyol/emulsifier for oil-based softgels or ointments.

β-Cyclodextrin Inclusion Complex: Soluble in cold/hot water in a short time to provide clear solutions without further solubilizing agents.

Stability:

Temperature: Stable is <= 60°C, prolonged heating > 80°C leads to oxidation of isoflavones and browning of powder.

pH 4.6-7.3; hydrolysis of isoflavone cores by strong acids/alkali.

Light Exposure: Stable in vacuum-sealed, light-proof packaging as a dry powder; aqueous/alcoholic solutions oxidize fast in light.

Hygroscopicity 4:1 crude powder > 10:1 > 8% > 20% high-purity Moisture absorption is positively correlated with polysaccharide content.

Storage: vacuum aluminum foil packing. ≤25°C, <60% humidity. Shelf life: 24 months.

Pre-treatment of Raw Material HPTLC authentication of Red Clover Extract Powder, White Clover and impurities; low temperature drying (40–45°C) to manage moisture ≤9%; pulverization to 20-30 mesh for better penetration of solvent.

Routes of extraction:

4:1 Crude (Water Extract): DI water, 70–75°C, two cycles of 1.5 h.

10:1, 8%/20% Standardized (Ethanol Extraction) 50% aqueous ethanol Closed system reflux 45-55°C Three cycles 2h + 1.2h + 1.2h

Filtration & Concentration: Multi-stage filtration to remove fibers and colloids. Vacuum concentration ≤60°C to 18–25% solid extract.

Refining, fractionating:

4:1 Powder: Directly spray-dried, so the polysaccharides are intact.

10:1 Powder: Removal of colloids by centrifugation at low temperature (0–5°C, 12h).

Standardized 8%/20%: Isoflavones are enriched by D101 macroporous resin gradient elution (35–50% ethanol); 20% spec is further chromatographed.

Drying: Spray drying of 4:1/10:1 (145–155°C intake, ≤72°C exit); low temperature vacuum drying (42–48°C) of 8%/20% to avoid degradation of isoflavones.

Derivative Process: Water soluble powder by high speed homogenization of β-Cyclodextrin encapsulating

Packaging & Testing: Light-proof vacuum aluminum foil bags, 25kg drums; HPLC/ICP-MS/GC-MS testing; complete COA/MSDS; third-party re-testing assistance.

Menopausal Health: Phytosterogenic modulation helps to reduce hot flashes, nocturnal sweats and mood swings.

Bone Density Support: Encourages osteoblast activity and calcium retention.

Heart Health: Supports cholesterol levels already within a healthy range and vascular elasticity.

Hormonal Balance: Light manipulation of estrogen receptors for women’s health.

Versatility of Formulation: Capsules, tablets, oral liquids, solid drinks, topical creams (with solubilizers).

Certifications: ISO9001, ISO22000, FSSC22000, HACCP, HALAL, KOSHER, cGMP, NOP, EOS, Non-GMO, Vegan.

Test Method: HPLC for isoflavone quantification; HPTLC for authentication; ICP-MS for heavy metals; GC-MS for pesticide/solvent residues; microbiological limit tests; complete COA/MSDS; third party validation available.

Traceability: Batch-to-batch traceability from culture bases to final product, with organic ingredients handled on separate lines.

Standard Packaging: 1kg aluminum foil bags; 25kg cartons or drums.

Custom Options: Tailored packaging for specialized transport protection.

Shipping: Worldwide air/sea freight; reinforced, light-proof, vacuum-sealed packaging ensures stability during high-humidity ocean transport.

Q. Are the raw ingredients from real Trifolium pratense (Red Clover)?

A: Yes. Only Red Clover flowers/leaves collected at peak bloom are used and fingerprinting by HPTLC is used to eliminate White Clover and pollutants. Materials are taken from standard bases, organic grades are processed on specialist lines.

Q: How are isoflavones tested?

A: Total and individual isoflavones (formononetin, genistein) determined by HPLC according to US/EU standards. With every batch, we provide COA and chromatograms that are verified by a third party.

Q: What is the difference between 4:1 and 8%/20% extracts for menopause products?

A: 4:1 is a cheap crude extract containing impurities, for budget mixes. 8% has a consistent content with a single step resin purification, and is perfect for mid-range capsules. 20% employs multi-stage enrichment in premium high potency formulas.

Q: Can the product be added to softgel oil bases?

A: Pure oils do not dissolve regular powder, it has to be dissolved with a polyol/emulsifier first. Cyclodextrin encapsulated, water soluble powder for direct dispersion in aqueous formulations.

Q: What's the temperature tolerance and storage sensitivity?

A: Stable at ≤60°C; degradation of isoflavones at >80°C. pH 4.6-7.3. Avoid strong acids/alkalis. 4:1 powder is quite hygroscopic; 8%/20% are not. Vacuum sealed and lightproof container, 24 month shelf life. Caking in high humidity during shipping is resisted.

Ready to enhance your formulations with premium Red Clover Extract Powder? contact us at information@sxrebecca.com for samples, COA, custom specifications, or OEM/ODM inquiries.