Rebaudioside M

PROPERTIES OF REBAUDIOSIDE M

Physicochemical properties

Reb M, which is also referred to as Reb X, has about 200–350 times the sweetness of sucrose with absolutely no calories . The organoleptic properties of Reb M are also in close resemblance to that of sucrose . Reb M, has a formular of C56H90O33 and molecular weight of 1291.3 g/mol. It is slightly soluble in water depicting a thermodynamic equilibrium solubility of 0.26% in water at 25°C . Reb M has its (Glcβ1-2(Glcβ1-3) Glcβ1-) linked at C13 and C19 of the aglycon steviol and may represent Reb A, in which position 2 and 3 hydroxyl groups of the beta d-glucosyl ester moiety have been transformed to a corresponding β-d-glucoside.

Stability of rebaudioside M

In its dried state and at ambient temperature with controlled humidity, Reb M may remain stable for a calendar year. Low pH greatly affects the stability of Reb M in solution; however, Reb is found to be stable at pH range of 4–8. Increase in temperature relatively decreases the stability of Reb M. The stability of Reb M has a great similarity to that of Reb A in various food application conditions. Similar human gastrointestinal hydrolysis may also occur for both Reb A and M . In general, the stability of Reb M meets industrial needs, hence their great use in commercial food and beverage productions .

Sweetness potency

Sweetness potency is measured by sucrose equivalency. The behavior of species both in aqueous and acidic media is of great importance in measuring sweetness potency. Reb M, a hexa-glycoside, has a property like sucrose; hence, considered one of the next generation sweeteners. Reb A has higher sweetness power (250–450 times the sweetness of sucrose) than Reb M (200–350 times the sweetness of sucrose), but Reb M has only a slightly bitter after taste unlike Reb A. Moreover, studies have revealed that Reb M gives sweeter perception in low pH water as well as decreased bitter lingering taste . Reb M has been established to be suitable for use in single and blended sweetening applications .

Manufacture Flow Chart

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The new proposed biosynthetic pathway for rebaudioside, including rebaudioside M in plant.

KINETIC AND METABOLISM OF REBAUDIOSIDE M

Generally, all SvGls (including Reb M) discovered so far have close chemical and structural similarities and exhibit similar metabolic fate once ingested. SvGls remain undigested in the upper gastrointestinal and gain direct access into the colon due to the lack of suitable digestive enzymes to hydrolyze the β-glycosidic bond . Moreover, SvGls are not susceptible to ready hydrolysis in in vitro studies by digestive enzymes, such as salivary α-amylase, pepsin, and pancreatic enzymes . However, certain communities of bacteria break down stevioside and Rebs A, B, D, E, and M to steviol due to the activity of their β-glucosidase to cleave β-glycosidic bond . The resulting steviol is taken up from the intestine and mobilized to the liver for metabolism. Steviol becomes conjugated with glucoronate in the liver leading to the formation of steviol glucuronide, which is then expelled as waste via the urine in humans and in feces in rats . The contrasting manner of excretion between species is due to the lower molecular weight threshold for biliary excretion in rats than in humans. In vitro experiments performed on pooled homogenates of human feces from healthy donors using Reb A, B, C, D, E, F, and M at two concentrations over a period of 24–48 h revealed that steviol was a most possible common metabolic product of all the different SvGls .

Reported the isolation and characterization of three degradation products of Reb M on the principle of considerable spectroscopic analysis sequel to its acidic degradation in 0.1 M phosphoric acid solution maintained at pH 2 and 80°C. The structures that were deduced on the basis of extensive chemical and spectral analysis, and enzymatic and hydrolytic studies include as follows: (a)13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy] ent-kaur-15-en-19-oic acid 2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl ester,(b)13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]-16β-hydroxy ent-kauran-19-oic acid 2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl ester, and (c) designated as 13-methyl-16-oxo-17-nor-ent-kauran-19-oic acid-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl ester. Taken together, there are few specific reports on the metabolism of Reb M unlike Reb A, which is the major SvGls in S. rebaudiana (Bertoni); therefore, such studies are required.

Typical rebaudioside M concentrations used to sweeten various foods and beverages

HEALTH BENEFITS OF REBAUDIOSIDE M

The intense sweetness and low impact of Reb M on blood sugar levels have gained appeal as a sugar alternative. SvGls has been linked to several health advantages aside from its sweetening qualities. Some of the possible health benefits of Reb M are antidiabetics, antihypertension, anticaries, and anticancer etc.

Packing

For powder products, we usually send the products together with cartons or fiber drums.Our packaging methods are 1 kg/aluminum bag, 25 kg/box, and 25 kg/barrel. For some products that require special packaging during transportation, we will carry out more detailed packaging.

Transportation

We support shipping by air, sea, FedEx, DHL, TNT, EMS, UPS, SF, and other carrier.

Our laboratory and Factory

As a professional plant extract supplier, our quality inspection department is equipped with the most advanced testing and identification instruments, such as UPLC, HPLC, UV and TT (active ingredients) GC and GC-MS (solvent residue), ICP-MS ( Heavy metals), GC/LC-MS-MS (pesticide residues), HPTLC and IR (identification), ELIASA (ORAC value), PPSL (irradiation residues), microbial detection, etc.

Qualification Certification

Shaanxi Rebecca Bio-Tech Co., Ltd. is certified by leading industry organizations, including:

GMP (Good Manufacturing Practice)

ISO 9001

Organic Certification (when applicable)

FAQ

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